# Antibody Titer Calculator AI Agent Connect

> Precision tool for calculating antibody titers, EC50, and concentrations from assay data.

## Overview
- **Category:** analytics
- **Price:** Free
- **Endpoint:** https://edge.vinkius.com/vk_preview_RFendKofbKD3RqCmjgTeLDk0lEm2K8npzWYGepVr/ai-agent-connect
- **Tags:** elisa, titer, ec50, immunoassay, biology

## Description

This MCP server provides analytical tools for immunoassays. It allows AI agents to process dilution series and signal measurements to determine critical metrics. Use `calculate_endpoint_titer` to find the limit of detection, `fit_dose_response_curve` for sigmoidal modeling, `calculate_antibody_concentration` for absolute quantification, and `validate_assay_quality` to ensure data reliability.

## Tools

### calculate_endpoint_titer
Determines the highest dilution factor that still yields a measurable signal above the noise

### fit_dose_response_curve
g., 4PL) to dilution and signal data.

Fits the experimental data to a sigmoidal model to derive curve characteristics

### validate_assay_quality
Evaluates if the provided dilution series is reliable enough for titration calculations

### calculate_antibody_concentration
Converts the calculated EC50 or endpoint into an absolute concentration value

## Prompt Examples

**Prompt:** 
```
What is the endpoint titer for dilutions [10, 100, 1000, 10000] with signals [0.5, 0.4, 0.1, 0.02] and a background of 0.01?
```

**Response:** 
```
The endpoint titer is 1000.
```

**Prompt:** 
```
Calculate the concentration for an EC50 of 500 using a standard factor of 0.5 in ng/mL.
```

**Response:** 
```
The calculated concentration is 250 ng/mL.
```

**Prompt:** 
```
Is my assay data reliable with signals [0.8, 0.75, 0.82] and a background of 0.05?
```

**Response:** 
```
Yes, the assay data is reliable with a high signal-to-noise ratio.
```

## Frequently Asked Questions

**How do I calculate the EC50?**
You can use the `fit_dose_response_curve` tool with your dilution and signal data to derive the EC50 value.

**Can I validate my assay data?**
Yes, the `validate_assay_quality` tool evaluates signal-to-noise ratios and data consistency.

**How is the endpoint titer determined?**
The `calculate_endpoint_titer` tool identifies the highest dilution factor that remains significantly above the background signal.