# DNA Concentration Calculator AI Agent Connect

> Calculate DNA concentration, purity, and yield from absorbance readings.

## Overview
- **Category:** science
- **Price:** Free
- **Endpoint:** https://edge.vinkius.com/vk_preview_6MYTb7bsP3NfUztfrvs5UaXewEJXZPsi6pc6WOqj/ai-agent-connect
- **Tags:** dna, concentration, purity, spectrophotometry, biotechnology

## Description

This MCP server provides specialized tools for nucleic acid quantitation. It allows AI agents to calculate DNA concentration using `get_dna_concentration`, determine sample purity via `get_purity_ratio`, assess quality with `get_purity_assessment`, and calculate total mass using `get_expected_yield`. It supports both double-stranded (dsDNA) and single-stranded (ssDNA) calculations based on A260 and A280 absorbance values.

## Tools

### get_dna_concentration
Calculates the concentration of DNA based on absorbance at 260nm and the structural form of the DNA

### get_expected_yield
Calculates the total mass of DNA present in a sample of a given volume

### get_purity_assessment
Provides a qualitative assessment of the DNA sample's purity based on the A260/A280 ratio

### get_purity_ratio
Determines the purity of the DNA sample by comparing the absorbance at 260nm to the absorbance at 280nm

## Prompt Examples

**Prompt:** 
```
What is the concentration of a dsDNA sample with an A260 of 0.5?
```

**Response:** 
```
The DNA concentration is 250.0 µg/mL.
```

**Prompt:** 
```
Calculate the purity ratio for a sample with A260=1.8 and A280=0.9.
```

**Response:** 
```
The purity ratio (A260/A280) is 2.0.
```

**Prompt:** 
```
If I have 50 µL of DNA at a concentration of 100 µg/mL, what is the total yield?
```

**Response:** 
```
The total yield is 5.0 µg.
```

## Frequently Asked Questions

**How do I calculate the DNA concentration?**
You can use the `get_dna_concentration` tool by providing the A260 absorbance reading and specifying if the DNA is 'dsDNA' or 'ssDNA'.

**How can I check if my DNA sample is pure?**
First, use `get_purity_ratio` to find the A260/A280 ratio, then use `get_purity_assessment` to receive a qualitative assessment of the sample quality.

**Can I calculate the total yield of my sample?**
Yes, use the `get_expected_yield` tool by providing the calculated concentration and the total sample volume in microliters (µL).