# Protein Concentration Calculator AI Agent Connect

> Calculate protein concentration and yield using UV280, colorimetric assays, or standard curves.

## Overview
- **Category:** biochemistry
- **Price:** Free
- **Endpoint:** https://edge.vinkius.com/vk_preview_Zwr9oMjvyjz3L7jgiY67BhDFpw5sLA0M8wEAdcyA/ai-agent-connect
- **Tags:** protein, spectrophotometry, biochemistry, concentration, lab-math

## Description

This MCP server provides specialized tools for quantifying protein concentration and total yield. It implements the Beer-Lambert law for direct UV280 measurements and provides linear regression logic for colorimetric assays like Bradford, Lowry, and BCA. Users can calculate molarity, mass concentration, and total yield from absorbance data or by generating a regression from a set of standard points.

## Tools

### calculate_colorimetric
Calculates protein concentration using colorimetric assays (Bradford, Lowry, or BCA)

### calculate_from_standard_curve
Determines concentration using a set of standard points to generate a regression

### calculate_uv280
Calculates protein concentration using direct UV absorbance at 280nm

### convert_units
Converts between different units of concentration and mass

## Prompt Examples

**Prompt:** 
```
Calculate the concentration for a protein with 0.5 absorbance at 280nm, an extinction coefficient of 43800, a molecular weight of 28000 Da, and a volume of 2 mL.
```

**Response:** 
```
The protein concentration is 0.026 mg/mL with a total yield of 0.052 mg.
```

**Prompt:** 
```
Using a Bradford assay with a slope of 0.05 and intercept of 0.01, what is the concentration for an absorbance of 0.4 and a volume of 5 mL?
```

**Response:** 
```
The mass concentration is 7.8 mg/mL and the total yield is 39.0 mg.
```

**Prompt:** 
```
I have standard points: (0, 0), (1, 0.2), (2, 0.4). What is the concentration for a sample with 0.3 absorbance and 1 mL volume?
```

**Response:** 
```
The mass concentration is 1.5 mg/mL and the total yield is 1.5 mg.
```

## Frequently Asked Questions

**Which assay types are supported?**
The server supports UV280 direct absorbance, Bradford, Lowry, and BCA colorimetric assays, as well as custom standard curve regressions.

**How do I calculate yield?**
The `calculate_uv280_concentration` and `calculate_colorimetric_concentration` tools automatically calculate the total yield by multiplying the mass concentration by the provided total volume.

**Can I convert between mg/mL and Molarity?**
Yes, use the `convert_units` tool. For mass-to-molar conversions, you must provide the molecular weight of the protein.